stillingsbetegnelse:
PhD position in Pharmaceutical Technology within the EU ITN project PIPPI - (ESR13)

jobbeskrivelse:
A 3-year PhD scholarship in Pharmaceutical Technology is available at the Department Pharmacy at the Ludwig-Maximilians-Universität München, Germany. The position will be available from 1st of June 2016 on or as soon as possible thereafter. The PhD student will be part of the EU-funded project “Protein-excipient Interactions and Protein-Protein Interactions in formulations” (PIPPI). Apart from the research work itself, the students’ participation in the project will involve tight collaboration with project partners and frequent visits to the involved labs, active participation at the bi-annual network events, as well as training in complementary skills such as project management. About PIPPI PIPPI is an Innovative Training Network (ITN) concerning the challenges in formulation of protein-based drugs. PIPPI will combine systematic investigations of the physicochemical behavior of a number of proteins with an in-depth understanding of the molecular interactions behind the macroscopic behavior. The overall objective is to develop methodologies, tools and databases to guide the formulation of robust biopharmaceuticals in the future. The Technical University of Denmark (DK) coordinates this exciting collaborative project. The academic partners include University of Manchester (UK), Ludwig-Maximilians-Universität, München (DE), Lund University (SE), University of Copenhagen (DK), MAXIV Laboratory (SE). The industry partners involved in the project include: Novozymes A/S (DK), MedImmune Ltd. (UK), Wyatt Technology Europe GmbH (DE), NanoTemper Technologies GmbH (DE). Each of the project partners contributes world-class competence in particular areas. The core research contribution to this project from the chair of Pharmaceutical Technology and Biopharmaceutics at LMU is in the area of research on stable formulations for modern protein drugs, establishing new formulation related protein analytics, fundamentals of understanding the role of protein and excipient molecules in the formulation and protein drug delivery. This PhD project (ESR13) focuses on the prediction of protein storage stability via folding stability determination. In general, we want to study effects of excipients and other formulation conditions on protein conformational stability and correlate the data with long term protein storage stability. So far, approaches for predicting protein storage stability with measuring protein unfolding or denaturation temperature lack correlation with real long term stability data on a relevant number of different proteins. Furthermore, different competing methods are used, each of them being sub-optimal regarding general applicability (e.g. in the presence of surfactants) and there is no consensus on a preferred standard. Most studies in the field correlate Tg data from high throughput methods (static and dynamic light scattering, differential scanning calorimetry, micro-scale thermophoresis, differential scanning fluorimetry) only with accelerated stability studies at higher temperature. More recently, chemical denaturation received more attention as a tool to predict protein stability because the most relevant room-temperature can be used during the analysis. We want to use a combination of the methods named above and correlate the data with real long term stability data from own work and the database created throughout the project. Finally we will evaluate the data quality of the different methods. We will then try to find best correlations for as much protein as possible, and try to determine rules about when predictivity will be high and in which cases it is low. Recommendations for a rationale use of such predictive methods shall conclude the project. Secondment The successful candidate will visit both academic and industrial partners during the project. This will ensure the buildup of a portfolio of interdisciplinary techniques relevant for the applicant’s project and PIPPI objectives. A particularly

Arbejdsgiver Navn: Danmarks Tekniske Universitet

Arbejdsgiver telefonnummer: 45252525

Arbejdsgiver faxnummer: 45881799

Job kort beskrivelse:
Undervisning og forskning på universiteter og højere læreranstalter

land: Danmark

region: Hovedstaden

krævede uddannelse: Ikke angivet

Kilde til jobtilbud: AMS, Offentlige arbejdsformidlinger, Danmark