Arbejde PhD scholarship: Mass spectrometry imaging (MSI) based investigation of interdependent agent-host responses in veterinary animals Hovedstaden Danmarks Tekniske Universitet - jobtilbud

GÆLDER
stillingsbetegnelse:
PhD scholarship: Mass spectrometry imaging (MSI) based investigation of interdependent agent-host responses in veterinary animals

jobbeskrivelse:
DTU Veterinary Institute (DTU Vet) is seeking a PhD student for a 3-year project on Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) and mass spectrometry imaging (MSI) development. Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is characterized by its rapid analysis time ‘shooting’ the Laser directly onto sample deposited on a sample plate, as well as by its wide mass range. It is also possible to use the method for the systematic analysis and the targeted tracing of peptides in a specially prepared tissue slide by the method of MALDI MS imaging (MSI). In essence the method allows the pseudostaining of both extraneous agent and associated host responses molecules, enabling the idenfication of bacteria and host response factors. The PhD project will focus on both basic MSI methods development, the application of MSI to understand the biology of a range of important veterinary infections and the use of the gained knowledge to demonstrate the usability of MSI to MRSA carrier detection in pigs. Specifically the project will address the possibility of using MSI to identify Foot-and-Mouth-Disease virus (FMDV) infected cattle at risk for becoming persistently infected from cattle, the MSI-based characterization of Clostridium perfringens toxin expression and intestinal host responses in the pig and pathogen and host responses in lung tissue of pigs experimentally infected with lung pathogens. In the final phase of the project peptides characteristic of methicillin-resistant Staphylococcus aureus (MRSA) will be identified by MSI and investigated for use as possible markers of MRSA-carriers in a pig herd. Responsibilities and tasks The overall goal of the project is to develop and apply MSI to characterize pathogen and host protein abundance in selected tissues of infected animals with the aim of developing diagnostic methods as well as gaining increased understanding of the coplex and interdependent control of protein expression in tissue from animals affected by infection. The PhD student will be responsible for planning and performing analytical studies based on experimental animal samples available at DTU Vet, including virus and bacterial experimental infection models in pigs and cattle. Methods to be employed and developed by the PhD student include MSI, optimization of tissue handling for MSI and diagnostic methods for animals at risk for becoming FMDV-carriers, and for identifying MRSA carriers in pig herds. Qualifications Candidates should have a master's degree in engineering or a similar degree with an academic level equivalent to the master's degree in engineering. Approval and Enrolment The scholarships for the PhD degree are subject to academic approval, and the candidates will be enrolled in one of the general degree programmes of DTU. For information about the general requirements for enrolment and the general planning of the scholarship studies, please see the DTU PhD Guide. Assessment The assessment of the applicants will be made by: Professor Peter Heegaard (DTU Vet, Section for Immunology and Vaccinology) and Section Head Ulrike Lyhs (DTU Vet, Section for Bacteriology, Pathology and Parasitology). We offer We offer an interesting and challenging job in an international environment focusing on education, research, public-sector consultancy and innovation, which contribute to enhancing the economy and improving social welfare. We strive for academic excellence, collegial respect and freedom tempered by responsibility. The Technical University of Denmark (DTU) is a leading technical university in northern Europe and benchmarks with the best universities in the world. Salary and appointment terms The salary and appointment terms are consistent with the current rules for PhD degree students. The period of employment is 3 years. Further information Further information may be obtained from Peter Heegaard, pmhh (at) vet.dtu.dk Please do not send applications to this e-ma

Arbejdsgiver Navn: Danmarks Tekniske Universitet

Arbejdsgiver telefonnummer: 45252525

Arbejdsgiver faxnummer: 45881799

Job kort beskrivelse:
Undervisning og forskning på universiteter og højere læreranstalter

land: Danmark

region: Hovedstaden

krævede uddannelse: Ikke angivet

Kilde til jobtilbud: AMS, Offentlige arbejdsformidlinger, Danmark

GÆLDER

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