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stillingsbetegnelse: Postdoc in Protein Science

jobbeskrivelse:

A 2-year postdoctoral fellowship is available at Department of Biotechnology and Biomedicine (DTU Bioengineering), the Technical University of Denmark. The position will be placed in the Enzyme and Protein Chemistry group in the section of Protein Chemistry and Enzyme Technology with about 70 staff members.

The postdoctoral position is part of the project 'WPAC. Structure and Design of Whey Protein Alginate Complexes' granted by the Novo Nordisk Foundation.

This research is carried out at and coordinated by DTU Bioengineering in collaboration with Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim; Department of Biology, University of Copenhagen; Center for Molecular Protein Science, Lund University; Department of Food Science, University of Copenhagen; DTU Chemistry; Arla Food Ingredients (AFI) and DuPont. The goal of the project is to advance insights into how the anionic polysaccharide hydrocolloid alginate interacts with important food proteins, such as whey proteins.

Responsibilities and tasks


The project work for this position includes production of recombinant b-lactoglobulin and analysis of binding sites interacting with defined alginate oligosaccharides using X-ray crystallography together with DTU Chemistry; enzymatic and chemical cross-linking of whey protein mixtures and identification of cross-linked amino acid residues using mass spectrometry and doing the data analysis with Lund University; analysis of protein-alginate or alginate oligosaccharide complex formation by biophysical techniques (SAXS, DLS, SEC) involving DTU Chemistry, and characterisation of surface properties of protein/alginate complexes as well as their in vitro digestion under simulated gastric and intestinal conditions with Department of Food Science at University of Copenhagen.

The project is multidisciplinary and based on collaboration with a group of experts who already collaborate on related research questions. On structural biology and biophysics of protein-alginate oligosaccharide complexes you will be working with P. Harris, G. Peters, J. Ø. Duus and K. Almdal, DTU Chemistry, F. L. Aachmann, Norwegian University of Science and Technology and B. B. Kragelund, SBINLab, Department of Biology, University of Copenhagen. Crosslinking studies and mass spectrometry analysis involves the proteomics platform at DTU Bioengineering and C. Emanuelsson at Department of Biochemistry, Lund University. Analysis of surface properties and in vitro simulated gastrointestinal tract digestion is done with R. Ipsen, Department of Food Science, University of Copenhagen. A PhD student in WPAC uses SPR and ITC for binding analysis with P. Westh, DTU Bioengineering, and is engaged in identification of binding sites on b-lactoglobulin of defined alginate oligosaccharides by NMR titration with SBINLab and NTNU. Several project students work in WPAC.

Qualifications

Candidates should have a PhD degree or equivalent.

We expect you have a solid background within protein science. Experience in particular with protein structure analysis (X-ray crystallography) as well as some of the below techniques will be an advantage

• Biophysical methods for measuring protein molecular interactions
• Recombinant production, purification and characterization of proteins
• Interactions of oligosaccharides / polysaccharides with proteins and enzymes
• Protein mass spectrometry
• Protein structure determination and molecular docking

We expect you are motivated for

• both individual and team accomplishments
• working in a multidisciplinary project
• interacting with international experts

We offer


DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and terms of employment


The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The main workplace will be at the DTU campus in Lyngby, 10 km north of Copenhagen, Denmark. There will be work visits to the partners in Copenhagen, Trondheim and Lund

The position is available from 11th March, 2019, or later as you are available.

You can read more about career paths at DTU here. Further information http://www.dtu.dk/english/about/job-and-career/working-at-dtu/career-paths

Further information may be obtained from Prof. Birte Svensson (DTU Bioengineering, tel.: +45 4525 2740).

You can read more about DTU Bioengineering on http://www.bioengineering.dtu.dk Application procedure http://www.bioengineering.dtu.dk/

Please submit your online application no later than 17 February 2019 (local time).

To apply, please open the link "Apply online", fill out the online application form. The following must be attached in English:

• Application (cover letter)
• CV
• Diploma (MSc/PhD)
• List of publications
• Up to three letters of recommendation

Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

Department of Biotechnology and Biomedicine (DTU Bioengineering) is an international leader in biotechnology and biomedicine. Our engineering approach to all aspects of biotechnology and biomedicine positions us as a valuable player with unique competences in a growing bio-based economy and with health systems transforming towards personalized medicine. We have a clear focus on the needs in the life science industry and point towards innovation, new businesses and scientific services for the benefit of society. The department has extensive collaborations with national and international research units and industry. DTU Bioengineering has approx. 190 employees, of which 2/3 is scientific staff. The department is located at DTU Lyngby Campus.

DTU is a technical university providing internationally leading research, education, innovation and scientific advice. Our staff of 6,000 advance science and technology to create innovative solutions that meet the demands of society, and our 11,200 students are being educated to address the technological challenges of the future. DTU is an independent academic university collaborating globally with business, industry, government and public agencies.


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Arbejdsgiver Navn: Danmarks Tekniske Universitet

source: DK-STAR