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stillingsbetegnelse: Postdoc in Bacterial Protein Phosphorylation

jobbeskrivelse:

DTU Biosustain announces a vacancy in the Bacterial Signal Transduction group headed by Professor Ivan Mijakovic. We are a bacteriology laboratory, working with a broad range of topics. One major focus of our research is protein phosphorylation, a reversible post-translational modification which forms the basis of many signaling and regulatory mechanisms in the bacterial cell. We study bacterial cell factories, employing phosphorylation systems to improve their yield, and bacterial pathogens, to exploit phosphorylation systems as targets for development of new antibacterial drugs.

This particular position is related to a basic science project on protein phosphorylation in the bacterium Bacillus subtilis. The project will be related to the question how relatively few kinases in bacteria can control the phosphorylation state of a large number of substrate proteins. The experimental approach will entail mass spectrometry-based quantitative phosphoproteomics which will lead into functional characterization, both in vitro and in vivo, of select phosphoproteins.

Responsibilities and tasks 
The successful candidate will be expected to independently conduct the experimental work in this project. This entails design, execution, and analysis of phosphoproteomics experiments. Functional characterisation of individual phosphorylation events will be conducted in vitro using proteins produced in and purified from E. coli. Verification in vivo will be done using e.g. phospho-mimetic and –ablative mutants in relevant physiological assays.

Qualifications
Candidates should have a PhD degree or equivalent.

For this project, we seek a researcher with experience in mass spectrometry-based quantitative proteomics (preferentially phosphoproteomics) as well as molecular biology (vector construction, protein synthesis in E. coli). Prior experience with research areas involving post-translational modifications would be considered an advantage. Experience with B. subtilis would be advantageous but not a requirement. Fluency in English, excellent communication and collaborations skills, as well as independence in paper and project writing are necessary prerequisites.

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and terms of employment
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The period of employment is for 2 years.

You can read more about career paths at DTU here

Further information 
Further information may be obtained from Carsten Jers cjer (at) biosustain.dtu.dk

You can read more about Novo Nordisk Foundation Center for Biosustainability on http://www.biosustain.dtu.dk ;

Application procedure
Please submit your online application no later than 7 January 2019 (local time). 

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

• Application (cover letter)
• CV
• Diploma (MSc/PhD)
• List of publications 

Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

DTU Biosustain is an international research center of excellence developing next generation cell factories and bioprocesses for sustainable production of high-value chemical compounds as well as protein-based products. The center uses advanced metabolic engineering techniques and computational biology ensuring efficient and cost-effective design and construction processes.
The center’s activities are a balanced mix of basic and translational research, complemented by an emphasis on business development to facilitate commercialization of new cell factories and associated technologies. DTU Biosustain offers state-of-the-art research facilities and assembles world leaders in the field thus offering a unique platform providing excellent talent development and career opportunities.

DTU is a technical university providing internationally leading research, education, innovation and scientific advice. Our staff of 6,000 advance science and technology to create innovative solutions that meet the demands of society, and our 11,200 students are being educated to address the technological challenges of the future. DTU is an independent academic university collaborating globally with business, industry, government and public agencies. 

numberOfPosts: 1

jobCategoriesCodes_0: http://data.europa.eu/esco/isco/C2310

abstractJV_contact_0: Please apply using one of the specified channels

positionTypeCode: directhire

contractTypeCodes_0: fulltime

Arbejdsgiver Navn: Danmarks Tekniske Universitet

source: DK-STAR